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Two assays reveal the birthplace of target proteins. In both, an antibody against a protein is used alongside an antibody that marks recent translation. The close proximity of both antibodies indicates the newly synthesized target protein.
CaptureSeq was used to quantitatively profile transcripts with low expression, resulting in a catalog of long noncoding RNA expression in 20 human tissues.
The fusion of three transcriptional activation domains to a nuclease-deficient Cas9 achieves robust induction of gene expression and can induce differentiation of hiPSCs.
New detector technology has improved the resolution of cryo-electron microscopy (cryo-EM), but tools for structure determination from high-resolution maps have lagged behind. Wang et al. describe a de novo approach for structure determination from high-resolution cryo-EM maps. Also in this issue, DiMaio et al. report structure determination using a homologous structure as a starting model.
FIB-SEM sample size is limited by cumulative milling artifacts and long imaging times.Ultrathick sectioning, followed by parallel FIB-SEM imaging and volume stitching,overcomes this limit, generating data sets of high quality for large-scale connectomics.
Two fluorescent reporters allow high-resolution visualization of auxin response; DR5v2 is more sensitive than existing auxin response reporters, and a ratiometric version of DII-Venus named R2D2 allows for accurate quantification of auxin input.
Real-time deformability cytometry allows the continuous mechanical characterization of cells with high throughput and is applied to distinguish cell-cycle phases, track differentiated cells and profile cell populations in whole blood.
Pareto task inference (ParTI) computes a polytype that encloses a data set and determines the enrichment of features around the vertices (archetypes) of the polytype, which allows the identification of the task the archetype represents.
The combination of molecularly tagging bacteria prior to infection and high-throughput sequencing of infected sites allows the quantitative assessment of the founding population size and analysis of bacterial migration patterns.
Binary expression systems such as the Gal4-UAS or Q-systems are useful tools for genetic manipulation in Drosophila. Here, improved reagents for the Q-system are described.
The fluorescent proteins mEos4a and mEos4b maintain their fluorescence and photoconversion after fixation with osmium. This property enables applications such as correlative super-resolution and electron microscopy.