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Kokubu and colleagues have developed a method for mapping cis-regulatory elements in the mouse using targeted integration of the Sleeping Beauty transposon. This method also gives researchers the ability to generate targeted deletions for testing loss-of-function effects.
Zsuzsanna Izsvák and colleagues report the generation of a hyperactive version of the Sleeping Beauty transposase that supports efficient and stable gene transfer into human CD34+ cells enriched for hematopoietic stem or progenitor cells.