Genetic manipulation of biosynthetic pathways is a useful method for producing analogs of complex bioactive metabolites, but this technique can be challenging when performed in the natural producer of the target compounds. Reconstruction of biosynthetic gene clusters in E. coli could be the key to rapid heterologous production of natural products and genetic manipulation of their biosynthetic pathways.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1038%2Fnchembio0806-398/MediaObjects/41589_2006_Article_BFnchembio0806398_Fig1_HTML.jpg)
Katie Ris
References
Walsh, C.T. ChemBioChem 3, 124–134 (2002).
Watanabe, K. et al. Nat. Chem. Biol. 2, 423–428 (2006).
Pfeifer, B.A., Admiraal, S.J., Gramajo, H., Cane, D.E. & Khosla, C. Science 291, 1790–1792 (2001).
Watanabe, K., Rude, M.A., Walsh, C.T. & Khosla, C. Proc. Natl. Acad. Sci. USA 100, 9774–9778 (2003).
Pfeifer, B.A., Wang, C.C.C., Walsh, C.T. & Khosla, C. Appl. Environ. Microbiol. 69, 6698–6702 (2003).
Gruenewald, S., Mootz, H.D., Stehmeier, P. & Stachelhaus, T. Appl. Environ. Microbiol. 70, 3282–3291 (2004).
Peirú, S., Menzella, H.G., Rodriguez, E., Carney, J. & Gramajo, H. Appl. Environ. Microbiol. 71, 2539–2547 (2005).
König, G.M., Kehraus, S., Seibert, S.F., Abdel-Lateff, A. & Müller, D. ChemBioChem 7, 229–238 (2006).
Schmidt, E.W. et al. Proc. Natl. Acad. Sci. USA 102, 7315–7320 (2005).
Wenzel, S.C. et al. Chem. Biol. 12, 349–356 (2005).
Mutka, S.C., Carney, J.R., Liu, Y. & Kennedy, J. Biochemistry 45, 1321–1330 (2006).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Challis, G. Engineering Escherichia coli to produce nonribosomal peptide antibiotics. Nat Chem Biol 2, 398–400 (2006). https://doi.org/10.1038/nchembio0806-398
Issue Date:
DOI: https://doi.org/10.1038/nchembio0806-398