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The jasmonoyl-isoleucine (JA-Ile) receptor COI1 is functionally conserved between the bryophyte Marchantia polymopha and the eudicot Arabidopsis thaliana, with two isomers of the JA-Ile precursor dinor-OPDA acting as the ligand for Marchantia COI1.
An ornithine–ammonia cycle involving an arginine dihydrolase was identified in cyanobacteria. This cycle serves as a conduit in the nitrogen storage-and-remobilization machinery and enables cellular adaptation to nitrogen fluctuations.
Crystal structures of a subunit of the ubiquitin ligase complex serving the N-end rule pathway of degrons marked by proline define a degron recognition mechanism and selection criteria for substrates.
Comprehensive glycome profiling of immunoglobulin G (IgG) in 95 strains of mice from the Collaborative Cross genetics resource reveals the extent and variability of IgG glycosylation in vivo.
Dissolved oxygen and a reducing-plus-oxidizing system suppress photobleaching and photoblinking in single-molecule tracking experiments, allowing long recordings of CD47 and integrin that showed temporary immobilization within focal adhesions.
The crystal structure and cryo-electron microscopy of the loading/condensing region of a nonreducing polyketide synthase reveals the insertion of a starter-unit acyltransferase into the condensing region and an asymmetrical post-loading state.
FINO2 is a small molecule that requires the endoperoxide moiety and hydroxyl group to promote ferroptosis through indirect inhibition of GPX4 enzymatic function and direct oxidation of iron, resulting in increased lipid peroxidation.
A single-molecule forced unfolding of E. coli chloride transporter ClC-ec1 shows that the N- and C-terminal halves of the protein unfold independently, with exposed polar surfaces stabilized by membrane lipid head groups and water.
The biosynthesis and secretion of redox-active coumarins sideretin and fraxetin in Arabidopsis thaliana enables the plant to acquire iron under nutrient-limited conditions and provides a blueprint for the use of related compounds in other eudicots.
The dTAG system pairs potent heterobifunctional degraders and extensible tagging strategies to achieve immediate and reversible degradation of divergent proteins, facilitating biological investigation and drug target validation in cells and in mice.
The source of biological toluene production in diverse anoxic microbial communities is a glycyl radical enzyme that catalyzes phenylacetate decarboxylation (PhdB), and its cognate activating radical S-adenosylmethionine enzyme (PhdA).
A photoswitchable diacylglycerol enabled a screen that found critical TRPC3 lipid-sensing residues and identified a lateral fenestration in the pore domain that allows lipids to protrude toward the permeation pathway to control channel gating.
The structure of a Stig cyclase, HpiC1, reveals how it catalyzes Cope rearrangement and 6-exo-trig cyclization, including how it controls the position of electrophilic aromatic substation that distinguishes hapalindole from fischerindole alkaloids.
A nonequilibrium thermodynamic model can explain how molecular chaperones such as GroEL can use the energy from ATP hydrolysis to maintain substrate proteins in an active state, even under conditions that favor the substrate’s inactive unfolded state.
Selective TRIM24 degradation is achieved by co-opting the VHL E3 ubiquitin ligase machinery. TRIM24 degradation outperforms bromodomain inhibition, with an enhanced antiproliferative effect in acute leukemia, a novel context of TRIM24 dependency.
Post-translational modification of residues in an intrinsically disordered region of Bcl-XL promotes interactions with its folded core and allosterically reduces affinity for proapoptotic BH3-domain-containing proteins, resulting in apoptosis.
The substrate-tolerant lanthipeptide synthetase ProcM enables the construction of a plasmid-encoded library of bicyclic lanthipeptides, from which an inhibitor of the p6–UEV protein–protein interaction is identified by a reverse two-hybrid screen.
Directed evolution of Trp repressor (TrpR) variants that are responsive to halogenated tryptophan analogs and recognize new operator sites serve as useful components for constructing complex gene expression networks.
Networking-associated genome mining on bacterial genomes followed by chemical and enzymatic analysis identified a mechanism of resistance toward nonribosomal peptide antibiotics based on hydrolytic cleavage by d-stereospecific peptidases.
Directed evolution of opsins via robotic high-content screening finds a fluorescent reporter of voltage that is simultaneously optimized for brightness, localization and voltage sensitivity and is applicable in three model systems.