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Moghadam et al. developed a CRISPR transcriptional repressor to silence MyD88 expression in vivo to modulate immune response against AAV gene therapy and septicaemia.
Massou et al. combine cell stretching, super-resolution imaging and single-protein tracking to investigate integrin-based mechanosensing in live cells.
Li and colleagues report base editor variants with improved targeting efficiencies and broader editing windows by fusing the original base editors with the single-stranded DNA-binding domain of Rad51.
Artegiani, Hendriks et al. describe a CRISPR–Cas9-based method to efficiently generate human knock-in organoids using non-homologous end joining to study rare intestinal cell types and human hepatocyte division.
Yue, Zong, Li, Li, Zhang, Wu et al. introduce an in toto live-imaging system to track cardiac ventricle chamber formation at single-cell resolution for up to 1.5 days and digitally reconstruct cell dynamics.
Kim et al. develop an optogenetic visualization approach that can rapidly and reversibly trap messenger RNA molecules in protein clusters, thereby restricting their access to ribosomes and dampening translation efficiency.