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He and colleagues develop itChIP-seq based on simultaneous cellular indexing and chromatin tagmentation. itChIP-seq is applicable to both low-input and single-cell analyses of chromatin states.
Simunovic et al. use human embryonic stem cells to generate a three-dimensional model of a human pre-gastrulation epiblast and show that anterior–posterior symmetry breaking can be induced by BMP4 and WNT signalling.
Coppé and colleagues design a peptide phosphorylation-screening system that simultaneously measures the enzymatic activity of multiple kinases, identifying mechanisms of therapy resistance and druggable targets in colorectal cancer and melanoma.
Gutnick et al. design a light-sensitive small molecule, zapalog, which reversibly dimerizes any two proteins. Tethering mitochondria to active kinesin motors uncovers distinct modes of mitochondrial motility in axons.
Wang et al. developed an inducible CRISPR–Cas9 system, in which guide RNA release is controlled by specific microRNAs, and demonstrated its application as a microRNA sensor and cell-type-specific genome regulator.
Nair et al. report the generation of human ESC-derived mature and functional β cells in vitro with a culture system including a step to induce clustering of immature β-like cells.