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Giulitti et al. deliver modified mRNAs encoding OCT3/4, SOX2, KLF4 and cMYC as well as NANOG in microfluidics to directly convert human fibroblasts into naive induced pluripotent stem cells; the confined environment leads to enhanced efficiency and homogeneity compared to traditional methods.
Harada et al. develop a chromatin integration labelling (ChIL) method to map distributions of histone modifications and DNA-binding factors at low-input or even single-cell levels.
Li and colleagues develop a CRISPR–Cas9-based screen strategy that combines base editing and haploid embryonic stem cell technologies to identify amino acids critical for protein function in mice.
Mehta et al. create single-fluorophore kinase activity sensors for PKA, PKC and ERK in different colours, and demonstrate that they enable multiplex imaging of a combination of signalling activities in cell lines and primary rat neurons.
Sozen et al. devise an approach to combine embryonic stem cells, trophoblast stem cells and extra-embryonic endoderm stem cells into self-assembling embryo-like structures, which recapitulate key hallmarks of gastrulation in vitro.
Mittasch et al. show that controlling cytoplasmic flow via focused-light-induced cytoplasmic streaming (FLUCS), a non-invasive technique, can be used to invert asymmetric cell division in Caenorhabditis elegans zygotes.
Ahier et al. describe a method to isolate intact mitochondria from specific cells in Caenorhabditis elegans and show that the germline is more prone to propagating deleterious mitochondrial genomes than somatic lineages.