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Amrute, Lai et al. performed single-nucleus RNA sequencing and compared the cellular and transcriptomic features of hearts from non-diseased donors, from patients with heart failure who recovered systolic function after left ventricular assist device implantation and from patients who did not recover. The analyses identified cell-type-specific signatures of recovery and revealed the downregulation of RUNX1 expression in macrophages and fibroblasts as a predictor of recovery, as confirmed by in silico simulations and re-analysis of data from a mouse model of cardiac functional recovery.
Berg Luecke et al. developed an analytical platform, CellSurfer, that enables the quantitative profiling of cell surface proteome (surfaceome) from small samples, and they apply it to primary human heart cells. The analyses revealed LSMEM2 as a surface protein specific for healthy cardiomyocytes; important surfaceome differences between primary human cardiac cells and the pluripotent stem cell derivatives; and differences in the abundance of surface proteins between human failing and non-failing cardiomyocytes.
Deviatiiarov, Gams et al. provide an atlas of the actively transcribed regulatory elements (promoters and enhancers) in the human heart by performing cap analysis of gene expression (CAGE) on 21 healthy donor hearts and ten failing hearts from ischemic and non-ischemic cardiomyopathy. The data show how the alternative use of regulatory elements modulates the differential gene expression across different heart chambers, disease states and disease types.
