Volume 1 Issue 11, November 2017

Nanoparticle delivery of CRISPR tools

This issue highlights the delivery of CRISPR components via nanoparticles, and advances in the precision of CRISPR/Cas9 gene editing, the modulation of global patterns in gene expression, the imaging of calcification in aortic valves, and the understanding of glial responses to implanted electrodes.

The cover illustrates the delivery of Cas9 ribonucleoprotein and donor DNA by gold nanoparticles, for the correction of a gene mutation (Article)

Image by Karen Moore.

Editorial

The expanding toolbox for genome engineering

The optimization and diversification of methods for manipulating the genome will enable new therapeutic solutions.

Collection:

Genome engineering
Editorial 10 Nov 2017
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News & Views

Nanoparticles for CRISPR–Cas9 delivery

The DNA mutation that causes Duchenne muscular dystrophy in mice can be corrected, with minimal off-target effects, by gold nanoparticles carrying the CRISPR components.

Zachary Glass
Yamin Li
Qiaobing Xu

Collections:

Genome engineering

Delivery of drugs and biologics
News & Views 10 Nov 2017
Enhanced precision and efficiency

The expression of two DNA repair factors improves the recombination of single-stranded oligodeoxynucleotides with Cas9-induced double-strand breaks, facilitating precise and efficient gene editing.

Sanum Bashir
Ralf Kühn

Collection:

Genome engineering
News & Views 10 Nov 2017
Repacking chromatin for therapy

A theoretical model of chromatin packing heterogeneity predicts patterns in gene expression and can be used to screen for effective chemotherapeutic adjuvants.

Alexandra Zidovska

Collection:

Genome engineering
News & Views 10 Nov 2017
Early detection of valvular calcification

The early stages of the formation of calcific nodules in the aortic valve can be detected by two-photon excited fluorescence microscopy.

Jack P. M. Andrews
Marc R. Dweck
News & Views 10 Nov 2017

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Reviews

Glial responses to implanted electrodes in the brain

This Review discusses the role of glia as an effector of the performance and integration of devices implanted in the brain, and the implications of this for device development.

Joseph W. Salatino
Kip A. Ludwig
Erin K. Purcell

Collections:

Bioelectronic interfaces

Technology for neuroengineering
Review Article 10 Nov 2017

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Research

Ectopic expression of RAD52 and dn53BP1 improves homology-directed repair during CRISPR–Cas9 genome editing

The expression of two specific DNA-repair factors promotes homology-directed repair and enhances the precision of CRISPR–Cas9 gene editing at multiple loci in human cells, including patient-derived induced pluripotent stem cells.

Bruna S. Paulsen
Pankaj K. Mandal
Derrick J. Rossi

Collections:

Genome engineering

Nobel Prize in Chemistry 2020
Article 09 Oct 2017
Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair

Gold nanoparticles carrying Cas9 ribonucleoprotein and donor DNA, and complexed with endosomal disruptive polymers, correct the DNA mutation that causes Duchenne muscular dystrophy in mice, with minimal off-target effects.

Kunwoo Lee
Michael Conboy
Niren Murthy

Collections:

Genome engineering

The Kavli Prize 2018

Nobel Prize in Chemistry 2020

Delivery of drugs and biologics
Article 02 Oct 2017
Macrogenomic engineering via modulation of the scaling of chromatin packing density

A model accounting for the properties of the local chromatin environment predicts the modulation of patterns in gene expression and helps screen for chemotherapeutic adjuvants that lead to an enhanced therapeutic response in cancer cells.

Luay M. Almassalha
Greta M. Bauer
Vadim Backman

Collection:

Genome engineering
Article 06 Nov 2017
Non-destructive two-photon excited fluorescence imaging identifies early nodules in calcific aortic-valve disease

A nonlinear microscopy method identifies a fluorescent signature consistent with the early signs of calcification in animal and human aortic valves.

Lauren M. Baugh
Zhiyi Liu
Irene Georgakoudi
Article 06 Nov 2017

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Volume 1 Issue 11, November 2017

Nanoparticle delivery of CRISPR tools

Editorial

The expanding toolbox for genome engineering

News & Views

Nanoparticles for CRISPR–Cas9 delivery

Enhanced precision and efficiency

Repacking chromatin for therapy

Early detection of valvular calcification

Reviews

Glial responses to implanted electrodes in the brain

Research

Ectopic expression of RAD52 and dn53BP1 improves homology-directed repair during CRISPR–Cas9 genome editing

Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair

Macrogenomic engineering via modulation of the scaling of chromatin packing density

Non-destructive two-photon excited fluorescence imaging identifies early nodules in calcific aortic-valve disease

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