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Pre-Clinical Studies

Evaluation of ex vivo expansion and engraftment in NOD-SCID mice of umbilical cord blood CD34+ cells using the DIDECO ‘Pluricell System’

Bone Marrow Transplantation volume 35, pages 1101–1106 (2005)Cite this article

Summary:

The Dideco ‘Pluricell System’ is a commercially available closed device composed of an expansion chamber and a kit of certified reagents that allow haematopoietic stem cell expansion. We have expanded seven umbilical cord blood (UCB) samples following the manufacturer's instructions; two groups of irradiated NOD-SCID mice have been transplanted with expanded and nonexpanded cells from the same UCB, and bone marrow was analysed for the presence of human cells. Average UCB volume was 61.6±8.8 ml; mean nucleated cell content was 1090.5±189.9 × 106. Percentage and number of CD34+ cells were 0.37±0.13% and 3.9±1.2 × 106. After separation, CD34+ cell purity was 82±11%. Mean number of inoculated cells was 760 000; mean NC and CD34+ fold expansion at 12 days was 230.4±91.5 and 21.0±11.9. Both groups of mice showed successful engraftment: the percentage of human cells was higher in the group receiving expanded cells (3.4±2.01%) compared to the group receiving nonexpanded cells (1.5±0.66%) (P<0.00018, Mann–Whitney test). The cell population obtained after 12 days expansion consisted mainly of myeloid and megakaryocytic progenitors. The CD34+ antigen reached the maximum expression level at day 12 (7.5±2.0%). Analysis of lineage-markers for human myelomonocytic, megakaryocytic, B, T, CD34 and erythroid cells, gave evidence that all the lineages were represented in the marrow of transplanted mice.

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Authors and Affiliations

  1. DIDECO srl, Mirandola, MO, Italy

    G Astori, G Mambrini, L Bigi & I Panzani

  2. SB Technology, Udine, Italy

    V Adami, E Falasca & W Malangone

  3. Servizio Modelli Animali, Istituto Nazionale Ricerca sul Cancro, Genova, Italy

    M Cilli

  4. Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti, Istituti Ortopedici Rizzoli, Bologna, Italy

    A Facchini

  5. DPMSC, Universita' degli Studi di Udine, Italy

    A Degrassi

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  1. G Astori
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Correspondence to G Mambrini.

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Astori, G., Adami, V., Mambrini, G. et al. Evaluation of ex vivo expansion and engraftment in NOD-SCID mice of umbilical cord blood CD34+ cells using the DIDECO ‘Pluricell System’. Bone Marrow Transplant 35, 1101–1106 (2005). https://doi.org/10.1038/sj.bmt.1704964

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