Conventional experiments for generating proteins with improved properties by directed evolution are iterative, lengthy and costly. Now, a label-free assay has been developed for ultrahigh-throughput microfluidic screening that can dramatically accelerate the discovery of superior biocatalysts from a single round of genetic randomization.
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Fessner, WD. How to meet the need for speed in protein evolution. Nat Catal 2, 738–739 (2019). https://doi.org/10.1038/s41929-019-0342-3
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DOI: https://doi.org/10.1038/s41929-019-0342-3