PROBER is a fast and sensitive episome-based method to identify sequence-specific DNA-binding proteins from living cells using proximity proteomics. This method quantifies steady-state and inducible association of transcription factors and corresponding chromatin regulators to specific DNA sequences as well as binding quantitative trait loci present as a result of single nucleotide variants.
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This is a summary of: Mondal, S. et al. PROBER identifies proteins associated with programmable sequence-specific DNA in living cells. Nat. Methods https://doi.org/10.1038/s41592-022-01552-w (2022).
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PROBER: overcoming the high noise of DNA–protein interactions studies. Nat Methods 19, 1044–1045 (2022). https://doi.org/10.1038/s41592-022-01553-9
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DOI: https://doi.org/10.1038/s41592-022-01553-9
