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GEMS enables mRNA m6A methylation sensing in living cells and m6A-coupled protein delivery

Nature Biotechnology (2024)Cite this article

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Thousands of cellular mRNAs contain the methyl modification m6A, which plays critical roles in mRNA processing and gene expression. We developed GEMS, a genetically encoded m6A sensor that provides a simple readout for m6A methylation in living cells and offers a platform for achieving m6A-coupled effector protein expression.

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Fig. 1: Schematic of the GEMS system for sensing cellular m6A.

References

  1. Flamand, M. F. et al. The proteins of mRNA modification: writers, readers, and erasers. Annu. Rev. Biochem. 92, 145–173 (2023). This review discusses the function and regulation of m6A and its links to physiology and disease.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Owens, M. C. et al. Recent technical advances in the study of nucleic acid modifications. Mol. Cell 81, 4116–4136 (2021). This review describes methods for detecting m6A and other RNA modifications.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Meyer, K. D. DART-seq: an antibody-free method for global m6A detection. Nat. Methods 16, 1275–1280 (2019). This paper describes the DART-seq method for transcriptome-wide m6A mapping.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Jiang, X. et al. The role of m6A modification in the biological functions and diseases. Signal Transduct. Target. Ther. 6, 74 (2021). This review discusses roles of m6A in physiology and disease.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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This is a summary of: Marayati, B. F., Thompson, M. G., Holley, C. L., Horner, S. M. & Meyer, K. D. Programmable protein expression using a genetically encoded m6A sensor. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01978-3 (2024).

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GEMS enables mRNA m6A methylation sensing in living cells and m6A-coupled protein delivery. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-023-01997-0

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