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Avoiding interpretational pitfalls in fluorescence imaging of the brain

Nature Reviews Neuroscience volume 23, pages 705–706 (2022)Cite this article

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Fluorescent sensors of molecular activity have revolutionized our knowledge of the brain. However, their signals report a reaction between the target and the sensor molecules rather than the activity of interest per se. Thus, understanding the location, sensitivity and imaging environment of a sensor should help to avoid misinterpretation of its readout.

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Fig. 1: Characteristic examples of fluorescence imaging settings prone to misinterpretation.

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  1. UCL Queen Square Institute of Neurology, University College London, London, UK

    Dmitri A. Rusakov

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  1. Dmitri A. Rusakov
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Correspondence to Dmitri A. Rusakov.

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Nature Reviews Neuroscience thanks the anonymous reviewer(s) for their contribution to the peer review of this work.

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Rusakov, D.A. Avoiding interpretational pitfalls in fluorescence imaging of the brain. Nat Rev Neurosci 23, 705–706 (2022). https://doi.org/10.1038/s41583-022-00643-z

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