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Membrane nanodiscs/single-molecule analysis

Lightly counting membrane proteins in native nanodiscs

Nature Nanotechnology volume 19, pages 5–6 (2024)Cite this article

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To determine the physiologically relevant oligomeric form of membrane proteins is extremely challenging. Now an elegant method of counting the oligomers in membrane proteins in near-native states is presented, using photobleaching and nanodiscs formed directly from cellular membranes.

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Fig. 1: Protein counting using the Native-nanoBleach method.

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Authors and Affiliations

  1. Department of Chemistry, The University of Chicago, Chicago, IL, USA

    Koushambi Mitra & Yamuna Krishnan

  2. Neuroscience Institute, The University of Chicago, Chicago, IL, USA

    Koushambi Mitra & Yamuna Krishnan

  3. Institute of Biophysical Dynamics, The University of Chicago, Chicago, IL, USA

    Koushambi Mitra & Yamuna Krishnan

Authors
  1. Koushambi Mitra
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  2. Yamuna Krishnan
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Correspondence to Yamuna Krishnan.

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The authors declare no competing interests.

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Mitra, K., Krishnan, Y. Lightly counting membrane proteins in native nanodiscs. Nat. Nanotechnol. 19, 5–6 (2024). https://doi.org/10.1038/s41565-023-01538-5

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