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Stem cell biology

A human bone marrow mesodermal-derived cell population with hemogenic potential

Leukemia volume 32, pages 1575–1586 (2018)Cite this article

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A Correction to this article was published on 11 June 2018

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Abstract

The presence, within the human bone marrow, of cells with both endothelial and hemogenic potential has been controversial. Herein, we identify, within the human fetal bone marrow, prior to establishment of hematopoiesis, a unique APLNR+, Stro-1+ cell population, co-expressing markers of early mesodermal precursors and/or hemogenic endothelium. In adult marrow, cells expressing similar markers are also found, but at very low frequency. These adult-derived cells can be extensively culture expanded in vitro without loss of potential, they preserve a biased hemogenic transcriptional profile, and, upon in vitro induction with Oct4, assume a hematopoietic phenotype. In vivo, these cells, upon transplantation into a fetal microenvironment, contribute to the vasculature, and generate hematopoietic cells that provide multilineage repopulation upon serial transplantation. The identification of this human somatic cell population provides novel insights into human ontogenetic hematovascular potential, which could lead to a better understanding of, and new target therapies for, malignant and nonmalignant hematologic disorders.

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  • 11 June 2018

    At the time of publication the funding information was omitted from the article – this has now been corrected in both the HTML and the PDF.

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Funding

This work was supported by NHLBI R01HL097623 and Intramural Pilot program of Wake Forest School of Medicine.

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Authors and Affiliations

  1. Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, 27157, USA

    Saloomeh Mokhtari, Weihong Yin, Zanetta Lamar, Steven Walker, Colin Bishop, Anthony Atala, Christopher D. Porada & Graça Almeida-Porada

  2. Animal Biotechnology, University of Nevada Reno, Reno, NV, 89557, USA

    Evan Colletti & Esmail D. Zanjani

  3. Currently at Fluidigm Corporation, San Francisco, CA, USA

    Chad Sanada

  4. Institute of Molecular Medicine, University of Texas at Houston, Houston, TX, 77030, USA

    Paul J. Simmons

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  1. Saloomeh Mokhtari
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Correspondence to Graça Almeida-Porada.

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Mokhtari, S., Colletti, E., Yin, W. et al. A human bone marrow mesodermal-derived cell population with hemogenic potential. Leukemia 32, 1575–1586 (2018). https://doi.org/10.1038/s41375-018-0016-1

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