Abstract
Magnesium sulfate is widely used as treatment for imminent eclampsia or to prevent preterm labor. Its use for the purpose of neuroprotection in preemies is controversial. We evaluated whether they might also have an effect on function of the erythrocytes. The aim of the presented study was to compare the selected erythrocyte membrane parameters including ATP concentration, the band 3 (prominent transmembrane protein of erythrocytes)amount, and phosphotyrosine content in erythrocyte membranes of preterm newborn whose mothers were treated or untreated with magnesium sulfate. The study group consist of preterm neonates (n-15) prenatally exposed to magnesium sulphate. Mean GA was 29,1 weeks (range 26–34) and mean birth weight was 1331g (range 650-1950). Control group included 20 infants (without prenatal Mg2+ administration). Mean gestational age was 30,78 weeks (range 26–34) and mean birth weight was 1392g (range 800-1800). In both groups, blood from umbilical cord vein was collected with citrate and than erythrocyte ghost were prepared. After birth, ATP concentration in erythrocytes of treated group was lower by 24% in relation to the control one (1.27± 0.44 mmol/g Hb) but after 24h we observed similar level in both groups (1.02±0.12 vs 1.09±0.19). The band 3 amount determined using the specific antibody was significantly decreased (50%) in magnesium-treated newborns during the studied period. However, after birth we observed the same phosphotyrosine level in both groups. P-Tyr amount was unchanged in magnesium-treated newborns after 24h of life, whereas increased by 20% in the control group. The presence of MgATP complex is necessary for a proper functioning of protein kinases. The administration of magnesium sulfate could intensify the phosphorylation of band 3 by tyrosine kinases thus increasing the glycolysis in erythrocytes. Supported by the grants No. 5P05E 09224 from the State Committee for Scientific Research, and No. 503 from the MU of Lodz.
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MEDICAL UNIVERSITY OF LODZ, NEUROCHEMICAL LABORATORY, POLAND
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Gulczynska, E., Kesiak, M., Talar, T. et al. 162 Effect of Maternal Magnesium Sulfate Administration on Band 3 Phosphorylation in Erythrocytes of Preterm Newborns. Pediatr Res 58, 382 (2005). https://doi.org/10.1203/00006450-200508000-00191
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DOI: https://doi.org/10.1203/00006450-200508000-00191