Abstract
Immune complexes of the IgA isotype are clearly implicated in the immunopatnogenesis of several disorders in the pediatric population particularly IgA nephropathy and Henoch-Schonlein disease. Therefore we have studied the interactions of IgA and human monocyte monolayer cultures. Secretory IgA derived from human breast milk and/or IgA derived from an EBV B cell line were used in these studies. Specific binding of IgA to monocyte membrane receptors was confirmed by 1) dose dependent inhibition of IgA-EA rosette formation (control 77±3%; % inhibition IgA 50 μg/ml-88±4, IgA 5 μg/ml-64±15T but not IgG. 2) Iodinated IgA Bound to monocyte monolayers. Binding was dependent on the number or monocytes, reached saturation with increasing amounts of 125I-IgA and could be inhibited by unlabelled IgA. Incubation of monocyte monolayers in the presence of increasing concentrations of secretory IgA and F(ab')2 anti-IgA resulted in a dose dependent increase of the oxidative burst (table). Neither IgA or anti-IgA alone nor incubation of IgG with anti-IgA had any effect on the oxidative burst. These studies indicate that human monocytes have a receptor for IgA and that specific activation of the monocytes may occur via these receptors.
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Padeh, S., Passwell, J. IgA RECEPTOR MEDIATED ACTIVATION OF HUMAN MONOCYTES. Pediatr Res 21 (Suppl 4), 315 (1987). https://doi.org/10.1203/00006450-198704010-00890
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DOI: https://doi.org/10.1203/00006450-198704010-00890