Abstract
Adherence of the intestinal epithelial cell (IEC) to its basement membrane may critically effect migration and differentiation of the IEC. We have quantitated attachment of IEC-6 cells, an established rat IEC line, to various ECM macromolecules. Cells (6.0×105/well) were added to wells coated with type I collagen (Col I), type IV collagen (Col IV), laminin (Ln), fibronectin (Fn), gel matrix derived from EHS sarcoma, which has been shown to support culture of differentiated epithelial cell (GM), or albumin (Al) and incubated for 4 h. The attached cells were washed, lysed and the nuclei were counted. After 4 h, the number of cells attached to GM (2.6×105) was 50-100% greater than for the other substrates (p < .001, ANOVA). GM: 2.7± 0.1×105; Cole IV: 1.95±0.4×105; Col I: 1.3±0.3×105; Fn: 1.4±0.2×105; Ln: 1.9±0.1×105; Al: 1.0±0.3×105. Phase contrast microscopy showed that the substrate had a pronounced effect on cell morphology: after 24 h cells on GM formed cylindrical arrays which penetrated the gel, whereas cells on all other substrates were polygonal and showed little aggregation. Culture of IEC on GM may be a useful model for intestinal development.
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Olson, A., Blau, H., Danna, D. et al. REGULATION OF INTESTINAL EPITHELIAL CELL ATTACHMENT AND GROWTH BY EXTRACELLULAR MATRIX (ECM). Pediatr Res 21 (Suppl 4), 274 (1987). https://doi.org/10.1203/00006450-198704010-00640
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DOI: https://doi.org/10.1203/00006450-198704010-00640