Abstract
Administration of high titer Influenza B virus to young mice results in increased permeability in hepatic mitochondrial (HM) membranes (Biochem Med 28:109, 1982). Since membrane functions are influenced by lipid composition (Can J Biochem 58:1091, 1980), the possibility of virally-induced changes in HM lipids was studied. 4-6 week-old male Balb C mice were given 12,800 hemag-glutination units of egg-adapted Influenza B Lee/40 virus or vehicle i.v., fasted, given ad lib access to water, and studied 36 hours later. HM lipids were extracted (Can J Biochem Phys 37:911 1959) and cyclohexane extracts were scanned from 220-280 nm at 5 nm intervals. O.D.'s were decreased across the entire spectrum-for example at 233 nm values were 1.18±0.21 for treated mice vs 0.67±0.06 for controls (p<0.040). The spectrum of total HM lipids is influenced by both neutral and polar lipids. Total HM cholesterol (C) was increased in treated mice:23±3 vs. 18±2μg C/mg protein (p<0.001). Phospholipid (PL)/C ratio was also increased in the treated mice 10.96±1.68 vs. 8.96±1.80 (p<0.017). HM sphingomyelin was decreased in treated animals - 2.06±1.80% of PL vs. 3.78±2.28(p<0.05); other PL classes and HM triglycerides were unaffected by virus treatment. The virally induced increase in HM C may affect membrane ATPase (Am J Physiol 242:H254, 1984) and respiratory function (J Biol Chem 259:9997, 1986), and may render HM membranes more susceptible to the injurious effects of agents such as aspirin involved in the pathogenesis of Reye's Syndrome.
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Larroya, S., Schwarz, K. INFLUENZA VIRUS ALTERS HEPATIC MITOCHONDRIAL LIPIDS IN A MOUSE MODEL OF REYE'S SYNDROME. Pediatr Res 21 (Suppl 4), 271 (1987). https://doi.org/10.1203/00006450-198704010-00625
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DOI: https://doi.org/10.1203/00006450-198704010-00625