Abstract
The role of chemotactic and random migration(RM) in ABE is not known. They were determined in 9 pts with ABE due to shigella or salmonella between days 3&5 of admission and 9 controls. One day prior to study each isolate was grown in medium 199 to generate specific chemotactic factors (SCF). Leukocytes were harvested from heparinized blood and 1.0×106PMNS were deposited on to 3 u millipore filter which was placed in the Boyden's chamber. The upper compartment of the chamber was filled with Hank's solution (HS) and the lower with SCF 100uL/mL of HS. Three simultaneous chambers were also set up with endotoxin activated serum (EAS), E. coli generated factors (ECF) and HS alone in the lower compartments. After 3hr's incubation filters were stained and cells which migrated to lower surface were counted. A ratio of migrated to total cells with SCE, EAS & ECF was termed as chemotactic index(CI) and RM with HS alone. In 4 pts studies were repeated after therapy. Mean (±1SD) values are presented (table): Mean CIs of patients with all the 3 factors were significantly lower than those of controls and significantly increased after therapy. RM was similar. Supression PMN chemotaxis may be one step in the pathogenesis of ABE. Counteracting it with drug/agents may improve the outcome.
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Khan, A., Varghese, M. & Evans, H. NEUTROPHIL (PMN) MIGRATION IN RESPONSE TO CHEMOTACTIC FACTORS GENERATED BY THE INVADING PATHOGENS IN ACUTE BACTERIAL ENTERITIS (ABE). Pediatr Res 21 (Suppl 4), 270 (1987). https://doi.org/10.1203/00006450-198704010-00618
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DOI: https://doi.org/10.1203/00006450-198704010-00618