Abstract
Tin protoporphyrin (SnP), an inhibitor of heme oxygenase, has been propossd for the treatment of neonatal hyperbilirubinemia. The neonate is physiologically chcOestatic, thus normal excretion of SnP and bilirubin (BR) may be impaired. To investigate the effects of SnP on serum BR fractions during cholestasis, we administered a single dose of SnP (100 μmoles/g) to rats rendered cholestatic by bile duct legation. Four groups of rats were studied, 1 control group with vehicle injection at time of ligation, and 3 experimental groups, with varying time of SnP injections. Ligation of all rats was at T=0, and sacrifice at T=72 hrs. BR fractions were measured by HPLC. Covalent bound bUirubin protein conjugates (BP) were measured by solvent precipitation.
There was no difference in the percent of total BR contributed by unconjugated and conjugated BR. Conclusions; 1. SnP does not appear to interfere with bilirubin conjugation while suppressing total BR levels. 2. Cholestasis does not inhibit SnP action. 3. The hyperbilirubinemia of cholestasis can be modified by SnP, suggesting clinical relevance.
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Felber, S., Rosenthal, P. & Henton, D. SERUM BILIRUBIN FRACTIONS IN CHOLESTATIC RATS TREATED WITH TIN PROTOPORPHYRIN. Pediatr Res 21 (Suppl 4), 267 (1987). https://doi.org/10.1203/00006450-198704010-00597
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DOI: https://doi.org/10.1203/00006450-198704010-00597