Abstract
Studies in a variety of gram negative bacteria have shown that outer membrane proteins play a major role in the adherence of the pathogen to the host epithelium, in resistance to phagocytic killing mechanisms, and as potential antigens. CP is a gram negative rod associated with a subset of pediatric and adult patients with gastritis and peptic ulcer disease. It has been shown that CP contains three immunogenic proteins with approximate molecular weights of 14-21 KD, 33KD and 55-70 KD (J. Med. Microbiol 22:57, 1986). The current study explored the hypothesis that these antigenic proteins of CP are contained within the outer membrane. Outer membrane proteins from a pediatric isolate of CP were extracted and purified using ultracentrifugation and sequential washings with sodium laurel sarcosinate. These were analyzed by SDS PAGE and shown to contain six major proteins with relative molecular weights of 66KD, 40KD, 31KD, 28KD, 27KD and 16KD. This is in marked contrast to C. jejuni which contains two major outer membrane proteins with molecular weights of 63KD and 45KD. Mice were intraperitoneally immunized on Day 1 and 14 with sublethal (105 CFU) doses of CP and sacrificed on Day 17. The serum was examined for antibody production specific for CP outer membrane proteins by immunoblot (Western blot) analysis. There appeared to be three major reactive protein bands of relative molecular weights of 66KD, 27KD and 16KD. These results suggest that the three immunogenic proteins of CP are present in the outer membrane. The role of these CP-specific antibodies, [specific for CP], in protecting the host from chronic CP infection is now being investigated.
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Czinn, S., Hupertz, V., Carr, H. et al. IMMUNOGENICITY OF CAMPYLOBACTER PYLORIDES (CP) OUTER MEMBRANE PROTEINS. Pediatr Res 21 (Suppl 4), 266 (1987). https://doi.org/10.1203/00006450-198704010-00594
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DOI: https://doi.org/10.1203/00006450-198704010-00594