Abstract
Steroid hormone synthesis and accumulation of mRNAs for the cholesterol side-chain cleavage enzyme (P450scc) and IGF-II occur in parallel in human granulosa cells stimulated with chorionic gonadotropin (hCG), folliclestimulating (FSH) or cyclic AMP (cAMP), in fetal adrenal cells stimulated with adrenocorticotropin (ACTH) or cAMP, or placental cells stimulated with cAMP (R.V. and W.L.M., Proc. Natl. Acad. Sci. USA, in press). However, the regulation of ICF-II in the testis is unknown. We detected IGF-II mRNA in Northern blots of RNA from fetal adrenal, placenta, liver, kidney, muscle and testis, but not ovary. In testes of 25 fetuses from 13 to 26 weeks gestation, the abundance of IGF-II mRNA closely followed the pattern shown for P450scc mRNA (R.V. and W.L.M., J.Clin. Endocrinol. Metab. 63:1145), with a peak at 14-15 weeks and diminishing thereafter. In 20 week gestation total testicular cells cultured with serum, 100 ng/ml hCG elicited no change in amounts of mRNAs for P450scc, P450c17 (17α-hydroxylase/17, 20 lyase) or IGF-II, while 1 mM cAMP increased P450scc and P450c17 mFNAs 10-fold, but did not change IGF-II mRNA. 100 ng/dl hCG increases P450scc and IGF-II mRNAs in cultured granulosa cells, and 1 mM cAMP induces accumulation of both mRNAs in fetal adrenal placenta and granulosa cells. Thus, in contradistinction to IGF-II mRNA in other steroidogenic tissues, fetal adrenal cells or placenta, fetal testicular IGF-II mRNA does not respond to factors stimulating steroid hormone synthesis, despite similar age-dependent regulation during gestation.
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Voutilainen, R., Miller, W. REGULATION OF mRNA FOR INSULIN-LIKE GROWTH FACTOR II (IGF-II) IN THE FETAL TESTIS. Pediatr Res 21 (Suppl 4), 255 (1987). https://doi.org/10.1203/00006450-198704010-00526
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DOI: https://doi.org/10.1203/00006450-198704010-00526