Abstract
A striking property of neural crest cells is their migratory behaviour. Eventually they aggregate in the gut. We investigated whether myenteric and submucous clusters of neural cells in the gut still can invade aneuronal gut.
Aneuronal chick gut (E4) in combination with innervated quail gut of different developmental stages (E6, E10, E12) was cultured on the chorioallantoic membrane of the chicken embryo: an in vivo culture system. We were able to demonstrate the presence of quail neural cells in chick gut using nucleolar differences of chick and quail cells (visualised with Feulgen DNA staining).
We observed that neuronal cells of quail gut are able to invade aneuronal chick gut.
Our results indicate that neural crest cell aggregation is not an irreversible process. We postulate that aneuronal segments of the gut produce a diffusible factor, which has a trophic effect on neural cells in neighbouring innervated gut.
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Carel Meijers, J., Tibboel, D., Van Der Kamp, A. et al. EVIDENCE FOR THE PRODUCTION OF TROPHIC FACTORS FOR NEURAL CELLS BY ANEURONAL SEGMENTS OF EMBRYONAL CHICK GUT. Pediatr Res 21 (Suppl 4), 218 (1987). https://doi.org/10.1203/00006450-198704010-00311
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DOI: https://doi.org/10.1203/00006450-198704010-00311