Abstract
Seventeen healthy infants age 9-15 mos received a 2-injection sequence of a PRP oligosaccharide conjugate vaccine. Sera and samples of nasal mucus obtained using saline washes (NW) were taken before the 1° and 2° injections and 1 mo and 6 mos after the 2°. A rise in total serum anti-PRP Ab [determined by radio-antigen binding (RAB)] occurred in 15 of 17. NW anti-PRP Ab developed in 6 of 17 as detected by RAB and in 8 of 17 as detected by an Ig class-specific immunosorbent assay (ELISA); these ELIS.A responses occurred as IgG (7 of 17), IgM (5 of 17), and IgA (4 of 17). Ab responses in NW were related to the magnitude of the serum Ab response: in 8 infants with post-2° serum Ab of <2 μg/ml, NW responses were detected in 0 of 8 by RAB and 1 of 8 by ELISA; in 9 infants with serum Ab >2 μg/ml, NW responses were detected in 6 of 9 by RAB and 7 of 9 by ELISA. At 6 mos after 2° injection, anti-PRP Ab was still detectable in NW in 6 of 16 subjects. Thus, a mucosal Ab component may contribute to the protective potential of conjugate vaccines.
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Zahradnik, J., Porcelli, S. & Anderson, P. MUCOSAL AND SERUM ANTIBODY (Ab) RESPONSES TO PARENTERAL IMMUNIZATION OF 1-YR-OLD INFANTS WITH H. INFLUENZAE B CAPSULAR (PRP) ANTIGEN CONJUGATED TO A DIPH IHERIA PROTEIN CARRIER. Pediatr Res 21 (Suppl 4), 338 (1987). https://doi.org/10.1203/00006450-198704010-01027
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DOI: https://doi.org/10.1203/00006450-198704010-01027