Abstract
We have recently discovered that an antigenically distinct rotavirus (group B rotavirus) is associated with gastroenteritis (GE) in rats and humans from Baltimore, MD. We now report the comparison of the genomes of the Baltimore strain of GBR (GBR-B) with the GBR strain associated with epidemic GE in China (GBR-C). GBR cDNA clones were prepared from fecal isolates of these viruses. Dot hybridizations with these cDNA clones demonstrated nucleic acid sequence homology among GBR-B, GBR-C and bovine and porcine isolates of GBR. Northern blot techniques defined specific genome segments responsible for hybridization among the GBR. We also examined the relatedness of GBR strains by means of one-dimensional (1-D) fingerprints with T1 RNase digests of genomic RNA. Conservation of terminal sequences has previously been observed between genomic segments of different strains of standard rotaviruses (group A rotavirus), and the analysis of these terminal sequences has been employed in the grouping of rotavirus strains. 1-D fingerprints of GBR strains indicated variability rather than conservation in the sequences at the ends of gene segments obtained from individual GBR. These studies document the relatedness of GBR strains from different animal species and geographic locations. We also find that GBR are not just antigenically and genetically distinct from group A rotaviruses: GBR display structural differences in their terminal genomic sequences not noted with GAR.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Eiden, J., McCrae, M., Theil, K. et al. GENETIC COMPARISON OF ANIMAL AND HUMAN GROUP B ROTAVIRUSES USING CLONED cDNA PROBES AND TERMINAL FINGERPRINT ANALYSIS. Pediatr Res 21 (Suppl 4), 323 (1987). https://doi.org/10.1203/00006450-198704010-00938
Issue Date:
DOI: https://doi.org/10.1203/00006450-198704010-00938