Abstract
We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens.
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Acknowledgements
We thank D. Holzer (European Molecular Biology Laboratory, Heidelberg) for help with MDCK cell culture and transfection, and A. Schrödel and M. Löhr (German Cancer Research Center, Heidelberg) for providing the BxPC3 spheroids. E.H.K.S. and F.P. acknowledge the Forschungsprogramm Optische Technologien der Landesstiftung Baden-Württemberg for financial support. M.M. was supported by a joint collaboration between Hamamatsu and the German Cancer Research Center (Project PA 11631).
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Supplementary information
Supplementary Fig. 1
SPIM and confocal microscopy of CHO cells. (PDF 141 kb)
Supplementary Fig. 2
SPIM deconvolution of a Medaka embryo. (PDF 148 kb)
Supplementary Fig. 3
Confocal microscopy of pancreatic tumor cells. (PDF 91 kb)
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Verveer, P., Swoger, J., Pampaloni, F. et al. High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy. Nat Methods 4, 311–313 (2007). https://doi.org/10.1038/nmeth1017
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DOI: https://doi.org/10.1038/nmeth1017
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