Abstract
Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.
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Acknowledgements
We thank P. B. Hackett (University of Minnesota) for providing SB cassettes.
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Supplementary information
Supplementary Fig. 1
Lethality of Stch homozygous mutants revealed by testcross analysis.
Supplementary Table 1
Summary of the trapped genes in this study.
Supplementary Table 2
Summary of additional transposition sites.
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Kitada, K., Ishishita, S., Tosaka, K. et al. Transposon-tagged mutagenesis in the rat. Nat Methods 4, 131–133 (2007). https://doi.org/10.1038/nmeth1002
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DOI: https://doi.org/10.1038/nmeth1002
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