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Spying on cGMP with FRET

A new carefully optimized and characterized genetically encoded fluorescent sensor for cyclic GMP (cGMP) has fast kinetics and properties that should make it an excellent compromise between sensitivity and specificity when compared to existing sensors.

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Figure 1: A FRET-based sensor for reporting cGMP dynamics in living cells.

Katie Ris

References

  1. Nikolaev, V.O., Gambaryan, S. & Lohse, M.J. Nat. Methods 3, 23–25 (2006).

    Article  CAS  Google Scholar 

  2. Honda, A. et al. Proc. Natl. Acad. Sci. USA 98, 2437–2442 (2001).

    Article  CAS  Google Scholar 

  3. Sato, M., Hida, N., Ozawa, T. & Umezawa, Y. Anal. Chem. 72, 5918–5924 (2000).

    Article  CAS  Google Scholar 

  4. Berridge, M.J., Bootman, M.D. & Roderick, H.L. Nat. Rev. Mol. Cell Biol. 4, 517–529 (2003).

    Article  CAS  Google Scholar 

  5. De Koninck, P. & Schulman, H. Science 279, 227–230 (1998).

    Article  CAS  Google Scholar 

  6. Tomida, T., Hirose, K., Takizawa, A., Shibasaki, F. & Iino, M. EMBO J. 22, 3825–3832 (2003).

    Article  CAS  Google Scholar 

  7. Landa, L.R., Jr. et al. J. Biol. Chem. 280, 31294–31302 (2005).

    Article  CAS  Google Scholar 

  8. Violin, J.D., Zhang, J., Tsien, R.Y. & Newton, A.C. J. Cell Biol. 161, 899–909 (2003).

    Article  CAS  Google Scholar 

  9. Baird, G.S., Zacharias, D.A. & Tsien, R.Y. Proc. Natl. Acad. Sci. USA 96, 11241–11246 (1999).

    Article  CAS  Google Scholar 

  10. Nagai, T., Yamada, S., Tominaga, T., Ichikawa, M. & Miyawaki, A. Proc. Natl. Acad. Sci. USA 101, 10554–10559 (2004).

    Article  CAS  Google Scholar 

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Jalink, K. Spying on cGMP with FRET. Nat Methods 3, 11–12 (2006). https://doi.org/10.1038/nmeth0106-11

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