Myllykangas, S. et al. Nat. Biotechnol. 29, 1024–1027 (2011).

Targeted resequencing can require lengthy enrichment protocols prone to sample loss. Myllykangas et al. provide an efficient alternative in which targets are captured directly on an Illumina flow cell by fixed probes that act as primers. Subsequent amplification creates substrates for conventional bridge amplification and sequencing. The method, called oligonucleotide-selective sequencing (OS-seq), has comparable sensitivity and uniformity to other enrichment methods and can be automated.