Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Commentary
  • Published:

Frontiers in peptide–MHC class II multimer technology

Nature Immunology volume 3, pages 887–889 (2002)Cite this article

Although tetramer technology has been wildly successful for examination of MHC class I–recognizing T cells, the same hasn't been true for MHC class II reagents. A recent workshop at the US National Institute of Allergy and Infectious Diseases was convened to address this.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Electron micrograph of a class I MHC tetramer.

(Courtesy of L. Teyton).

Figure 2: Flexible multivalent pMHC class II reagents.

References

  1. Altman, J.D. et al. Science 274, 94–96 (1996).

    Article  CAS  PubMed  Google Scholar 

  2. Greten, T.F. et al. Proc. Natl. Acad. Sci. USA 95, 7568–7573 (1998).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Doherty, P.C. & Christensen, J.P. Annu. Rev. Immunol. 18, 561–592 (2000).

    Article  CAS  PubMed  Google Scholar 

  4. Casares. S. et al. Nature Immunol. 3, 383–391 (2002).

    Article  CAS  Google Scholar 

  5. Reijonen, H. et al. Diabetes 51, 1375–1382 (2002).

    Article  CAS  PubMed  Google Scholar 

  6. Nepom, G.T. et al. Arthritis Rheum. 46, 5–12 (2002).

    Article  CAS  PubMed  Google Scholar 

  7. McMichael, A.J. & Kelleher, A. J. Clin. Invest. 104, 1669–1670 (1999).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Crawford, F., Kozono, H., White, J., Marrack, P. & Kappler, J. Immunity 8, 675–682. (1998).

    Article  CAS  PubMed  Google Scholar 

  9. Latek, R.R., Petzold, S.J. & Unanue, E.R. Proc. Natl. Acad. Sci. USA 97, 11460–11465 (2000).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Livingston, B. et al. J. Immunol. 168, 5499–5506 (2002).

    Article  CAS  PubMed  Google Scholar 

  11. Sidney, J. et al. Hum. Immunol. 62, 1200–1216 (2001).

    Article  CAS  PubMed  Google Scholar 

  12. Rudolph, M.G., Luz, J.G. & Wilson, I.A. Annu. Rev. Biophys. Biomol. Struct. 31, 121–149 (2002).

    Article  CAS  PubMed  Google Scholar 

  13. Ober, B.T. et al. Int. Immunol. 12, 1353–1363 (2000).

    Article  CAS  PubMed  Google Scholar 

  14. Murali-Krishna, K. et al. Immunity 8, 177–187 (1998).

    Article  CAS  PubMed  Google Scholar 

  15. McMichael, A.J. & O'Callaghan, C.A. J. Exp. Med. 187, 1367–1371 (1998).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Bromley, S.K. et al. Annu. Rev. Immunol. 19, 375–396 (2001).

    Article  CAS  PubMed  Google Scholar 

  17. Knabel, M. et al. Nature Med. 8, 631–637 (2002).

    Article  CAS  PubMed  Google Scholar 

  18. Stone, J.D., Cochran, J.R. & Stern, L.J. Biophys. J. 81, 2547–2557 (2001).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Fahmy, T.M., Bieler, J.G., Edidin, M. & Schneck, J.P. Immunity 14, 135–143 (2001).

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank D. Margulies, H. Quill, S. Plaeger, K. Kraemer, S. McCarthy and D. Rotrosen for helpful advice and critical review of the manuscript.

Author information

Authors and Affiliations

  1. Division of Allergy, Immunology and Transplantation, National Institutes of Health, Bethesda, 20817, MD, USA

    Charles J. Hackett

  2. Division of Acquired Immunodeficiency Syndrome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, 20817, MD, USA

    Opendra K. Sharma

Authors
  1. Charles J. Hackett
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Opendra K. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Charles J. Hackett or Opendra K. Sharma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hackett, C., Sharma, O. Frontiers in peptide–MHC class II multimer technology. Nat Immunol 3, 887–889 (2002). https://doi.org/10.1038/ni1002-887

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/ni1002-887

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing