Although much is known about the molecular components of cellular signaling pathways, very little is known about how these multicomponent biochemical machineries process complex extracellular signals to generate a consolidated cellular response. A newly developed theoretical approach for reverse engineering network structure—analyzing how perturbations propagate in a network—can be combined with chemical perturbations and quantitative detection approaches to reveal the causal connections within protein networks in cells. This information indicates the dynamic capabilities of a network and thereby its potential function.
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Acknowledgements
E.Z. is supported by the European Union integrated project grant 'Interaction Proteome'.
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Zamir, E., Bastiaens, P. Reverse engineering intracellular biochemical networks. Nat Chem Biol 4, 643–647 (2008). https://doi.org/10.1038/nchembio1108-643
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DOI: https://doi.org/10.1038/nchembio1108-643
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