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Memorizing spatiotemporal patterns

Nature Chemical Biology volume 3, pages 598–601 (2007)Cite this article

Live samples are intrinsically highly dynamic, yet techniques to monitor these complex environments usually reflect snapshots, thus making time-lapse imaging necessary to explore temporal progression of biological functions. Recent results indicate that exploiting some basic features of fluorescent protein maturation, such as green-to-red maturation of engineered proteins, should allow probing of temporally regulated information.

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Figure 1: A comparison of schemes for the formation and maturation of chromophores in fluorescent proteins.
Figure 2: Principle of the bimolecular fluorescence complementation assay.
Figure 3

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Acknowledgements

The authors thank H. Mizuno and T. Kogure for preparation of figures and D. Mou for critical reading of the manuscript.

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Authors and Affiliations

  1. Atsushi Miyawaki is in the Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan, and the Life Function and Dynamics Project, Exploratory Research for Advanced Technology, JST, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan. matsushi@brain.riken.jp,

    Atsushi Miyawaki

  2. Satoshi Karasawa is in the Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan and Amalgaam Co., Ltd., 2-9-3 Itabashi, Itabashi-ku, Tokyo 173-0004, Japan.,

    Satoshi Karasawa

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Miyawaki, A., Karasawa, S. Memorizing spatiotemporal patterns. Nat Chem Biol 3, 598–601 (2007). https://doi.org/10.1038/nchembio1007-598

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