Identifying the structures of transient intermediates is an essential step in the elucidation of an enzymatic reaction mechanism. Cryocrystallography reveals the structures of three thiamine diphosphate derivatives as intermediates in the action of pyruvate oxidase.
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Frey, P. Snapshots of three intermediates at the active site of pyruvate oxidase. Nat Chem Biol 2, 294–295 (2006). https://doi.org/10.1038/nchembio0606-294
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DOI: https://doi.org/10.1038/nchembio0606-294