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DNA replication

Unlocking the secrets of fork arrest

Nature Chemical Biology volume 11, pages 550–551 (2015)Cite this article

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A powerful, high-throughput single-molecule approach to probe the nanoscale mechanical properties of the Tus–Ter protein–DNA complex reveals that the Tus–Ter-induced lock in unzipping at the nonpermissive face requires only DNA strand separation and involves a progressive strengthening of the Tus–Ter complex.

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Figure 1: Schematic depiction of Tus protein locked onto Ter site in the nonpermissive orientation owing to binding to the flipped-out C6 base.

Katie Vicari/Nature Publishing Group

References

  1. Buc, H. & Strick, T.R. in RNA Polymerases as Molecular Motors (Royal Society for Chemistry, 2009).

    Book  Google Scholar 

  2. Mulcair, M.D. et al. Cell 125, 1309–1319 (2006).

    Article  CAS  Google Scholar 

  3. Bastia, D. et al. Proc. Natl. Acad. Sci. USA 105, 12831–12836 (2008).

    Article  CAS  Google Scholar 

  4. Berghuis, B.A. et al. Nat. Chem. Biol. 10.1038/nchembio.1857 (6 July 2015).

  5. Pandey, M. et al. Nucleic Acids Res. doi:10.1093/nar/gkv527 (24 May 2015).

  6. Duggin, I.G., Wake, R.J., Bell, S.D. & Hill, T.M. Mol. Microbiol. 70, 1323–1333 (2008).

    Article  CAS  Google Scholar 

  7. Bailey, R., Moreno, S.P. & Gambus, A. Nucleus doi:10.1080/19491034.2015.1035843 (2 April 2015).

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Authors and Affiliations

  1. Jun Fan and Terence R. Strick are at the Institut Jacques Monod, Centre National de la Recherche Scientifique and University of Paris Diderot and Sorbonne Paris Cité, Paris, France.,

    Jun Fan & Terence R Strick

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  1. Jun Fan
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  2. Terence R Strick
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Correspondence to Terence R Strick.

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The authors declare no competing financial interests.

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Fan, J., Strick, T. Unlocking the secrets of fork arrest. Nat Chem Biol 11, 550–551 (2015). https://doi.org/10.1038/nchembio.1860

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