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Redox State of Single Chain Fv Fragments Targeted to the Endoplasmic Reticulum, Cytosol and Mitochondria

Bio/Technology volume 13, pages 1110–1115 (1995)Cite this article

Abstract

In this paper we have engineered the targeting of ScFv fragments to mitochondria and demonstrated that this can occurr efficiently. This extends the range of subcellular compartments where antibody domains can be targeted in order to interfere with the action of the corresponding antigen. Moreover, we have compared the redox state of ScFv fragments targeted to the secretory compartment, the cytosol and the mitochondria, and demonstrated that cysteine residues in ScFv targeted to the secretory compartments and to the mitochondria are oxidized. On the contrary, cytosolic antibody domains are expressed in a reduced state, which is probably the reason for their lower expression levels. These pittfalls, however, do not prevent their successful utilization for intracellular immunization.

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Authors and Affiliations

  1. Institute of Neurobiology, CNR, Viale K. Marx 15, 00137, Roma, Italy

    Silvia Biocca, Marco Tafani & Antonino Cattaneo

  2. Institute of Cell Biology, CNR, Viale K. Marx 15, 00137, Roma, Italy

    Paola Pierandrel-Amaldi

  3. International School for Advanced Studies (SISSA), Via Beirut 2-4, 34013, Trieste, Italy

    Francesca Ruberti & Antonino Cattaneo

Authors
  1. Silvia Biocca
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  2. Francesca Ruberti
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  3. Marco Tafani
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  4. Paola Pierandrel-Amaldi
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  5. Antonino Cattaneo
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Correspondence to Silvia Biocca or Antonino Cattaneo.

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Biocca, S., Ruberti, F., Tafani, M. et al. Redox State of Single Chain Fv Fragments Targeted to the Endoplasmic Reticulum, Cytosol and Mitochondria. Nat Biotechnol 13, 1110–1115 (1995). https://doi.org/10.1038/nbt1095-1110

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