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High Level Expression of a Chimeric Anti–Ganglioside GD2 Antibody: Genomic Kappa Sequences Improve Expression in COS and CHO Cells

Bio/Technology volume 10, pages 1121–1127 (1992)Cite this article

Abstract

We report a flexible strategy for the high level expression of a recombinant human monoclonal antibody (mAb) in Chinese hamster ovary (CHO) cells, initially using COS monkey kidney cell transfections to evaluate rapidly modifications to immunoglobulin (Ig) DNA constructs. Using sequential transfections with two amplifiable markers, we generated CHO cell lines and clones that secrete 80–110 μg/106 cells/24 hours of a mousehuman chimeric IgG1κ mAb. This cellular productivity is considerably greater than most murine hybridomas and transfected myelomas. Our data also demonstrate that genomic kappa sequences can improve mAb expression in COS and CHO cells. As a paradigm, we focused our expression studies on a human chimeric form of 3F8, a murine mAb that binds to ganglioside GD2 on neuroblastoma and melanoma tumor cells.

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  1. Lynette A. Fouser: Corresponding author.

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  1. Genetics Institute, 87 Cambridge Park Drive, Cambridge, Massachusetts, 02140

    Lynette A. Fouser, Stephen L. Swanberg, Bor-Yaw Lin, Mary Benedict, Kerry Kelleher, Dale A. Cumming & Gerard E. Riedel

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Fouser, L., Swanberg, S., Lin, BY. et al. High Level Expression of a Chimeric Anti–Ganglioside GD2 Antibody: Genomic Kappa Sequences Improve Expression in COS and CHO Cells. Nat Biotechnol 10, 1121–1127 (1992). https://doi.org/10.1038/nbt1092-1121

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