Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Research Paper
  • Published:

Sex Determination of Bovine Embryos by Restriction Fragment Polymorphisms of PCR Amplified ZFX/ZFY Loci

Bio/Technology volume 10, pages 805–807 (1992)Cite this article

Abstract

We have obtained the DNA sequence of a region from the bovine ZFY and ZFX loci and used it to design nested primers that, together with a previously described PstI polymorphism, were applied in a sensitive PCR assay to determine the sex of embryo biopsies.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Krlich, H.A., Gelfand, D. and Sninsky, J.J. 1991. Recent advances in polymerase chain reaction. Science 252: 1643–1651.

    Article  Google Scholar 

  2. Bondioli, K.R., Ellis, S.B., Pryor, J.H., Williams, M.W. and Harpold, M.M. 1989. The use of male-specific chromosomal DNA fragments to determine the sex of bovine preimplantation embryos. Theriogenology 31: 95–104.

    Article  Google Scholar 

  3. Herr, C.M., Matthaei, K.I., Petrzak, U. and Reed, K.C. 1990. A rapid Y-chromosome-detecting ovine embryo sexing assay. Theriogenology 33: 245.

    Article  Google Scholar 

  4. Herr, C.M., Holt, N.A., Matthaei, K.I. and Reed, K.C. 1990. Sex of progeny from bovine embryos sexed with a rapid Y-chromosome-detecting assay. Theriogenoiogy 33: 247.

    Article  Google Scholar 

  5. Bradbury, M.W., Isola, L.M. and Gordon, J.W. 1990. Enzymatic amplification of a Y chromosome repeat in a single blastomere allows identification of the sex of preimplantation mouse embryos. Proc. Natl. Acad. Sci. USA 87: 4053–4057.

    Article  CAS  Google Scholar 

  6. Handyside, A.H., Kontogianni, E.H., Hardy, K. and Winston, R.M.L. 1990. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature 344: 768–770.

    Article  CAS  Google Scholar 

  7. Scheneider Gadicke, A., Beer-Romero, P., Brown, L.G., Nussbaum, R. and Page, D.C. 1989. ZFX has a gene structure similar to ZFY, the putative human sex determinant, and escapes X inactivation. Cell 57: 1247–1258.

    Article  Google Scholar 

  8. Aasen, E. and Medrano, J.F. 1990. Amplification of the ZFY and ZFX genes for sex identification in humans, cattle, sheep and goats. Bio/Technology 8: 1279–1281.

    CAS  PubMed  Google Scholar 

  9. Sarkar, G. and Sommer, S.S. 1990. Shedding light on PCR contamination. Nature 334: 27.

    Article  Google Scholar 

  10. Maniatis, T., Fritsch, E.F. and Sambrook, J. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, NY.

  11. Sanger, F., Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463–5467.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Alberto C. C. Frasch: Corresponding author.

Authors and Affiliations

  1. Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405, Buenos Aires, Argentina

    Guido D. Pollevick, Alberto C. C. Frasch & Rodolfo A. Ugalde

  2. Tecnología Genética, Buenos Aires, Argentina

    Guido D. Pollevick & Susana Giambiagi

  3. International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34012, Trieste, Italy

    Susana Giambiagi, Sabrina Mancardi & Oscar Burrone

  4. Cabaña la Sorpresa, Torquinst, Argentina

    Leonardo De Luca

Authors
  1. Guido D. Pollevick
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Susana Giambiagi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sabrina Mancardi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Leonardo De Luca
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Oscar Burrone
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Alberto C. C. Frasch
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rodolfo A. Ugalde
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pollevick, G., Giambiagi, S., Mancardi, S. et al. Sex Determination of Bovine Embryos by Restriction Fragment Polymorphisms of PCR Amplified ZFX/ZFY Loci. Nat Biotechnol 10, 805–807 (1992). https://doi.org/10.1038/nbt0792-805

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0792-805

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing