The manual sorting of thousands of Drosophila embryos necessary to isolate homozygous mutants is both laborious and time-consuming. On page 153, Furlong et al. provide a solution, constructing a fluorescence-activated embryo sorter based on embryo expression of green fluorescent protein (GFP). The machine can automatically sort 15 embryos/s with better than 99% accuracy and has been adapted to sort embryos of other species as well. In addition, Furlong et al. have made available online the detailed plans for their machine.
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Jamison, J. Flies get sorted. Nat Biotechnol 19, 100 (2001). https://doi.org/10.1038/84318
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DOI: https://doi.org/10.1038/84318