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Transplanting Two Unique β-Glucanase Catalytic Activities Into One Multienzyme, Which Forms Glucose

Bio/Technology volume 14, pages 71–76 (1996)Cite this article

Abstract

Endo cellulases of plant pathogenic erwinias degrade cellulose as well as the cellulosic domains of barley (1-3,1-4)-β-glucan. Depolymerization of the latter substrate is mainly caused by (1-3,1-4)-β-glucanases, which hydrolyze (1-4)-β glycosidic linkages adjacent to (1-3)-β linkages. To construct an enzyme for efficient degradation of barley (1-3,1-4)-β-glucan, the sequence encoding the catalytic domain and interdomain linker of the cellulase from Erwinia carotovora subspecies atroseptica was fused to that for the heat stable Bacillus hybrid, H(A12-M)ΔY13 (1-3,1-4)-β-glucanase. The chimeric enzyme secreted from Escherichia coli cells did not remain covalently assembled as judged by SDS-PAGE. However, the glycosylated and intact enzyme (denoted CELGLU) is secreted from the yeast Pichia pastoris. CELGLU exhibits both cellulase and (1-3,1-4)-δ-glucanase catalytic activities, and was accordingly classified a true multienzyme. HPLC and NMR analyses revealed that among the products from CELGLU, di- and trimeric oligosaccharides were identical to those produced by the parental cellulase. Tetrameric oligosaccharides, derived from the (1-3,1-4)-β-glucanase activity of CELGLU, were further degraded by the cellulase moiety to yield glucose and trimers. Compared with the parental enzymes, CELGLU exhibits substantially higher Vmax for degradation of both soluble cellulose and barley (1-3,1-4)-β-glucan. These findings point to construction of multienzymes as an effective approach for engineering enzymes with novel characteristics.

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Authors and Affiliations

  1. Department of Physiology, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Copenhagen, Denmark

    Ole Olsen, Karl K. Thomsen, Jürgen Weber & Diter von Wettstein

  2. Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Copenhagen, Denmark

    Jens Ø Duus & Ib Svendsen

  3. Institute for Stress Physiology, Federal Centre for Breeding Research on Cultivated Plants, Institutsplatz 1, D-18190, Gross Lüsewitz, Germany

    Christina Wegener

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Olsen, O., Thomsen, K., Weber, J. et al. Transplanting Two Unique β-Glucanase Catalytic Activities Into One Multienzyme, Which Forms Glucose. Nat Biotechnol 14, 71–76 (1996). https://doi.org/10.1038/nbt0196-71

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