Papers from the literature selected by the Nature Biotechnology editors. (Follow us on Twitter, @NatureBiotech #nbtHighlight)

Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system

Zetsche, B. et al. Cell doi:10.1016/j.cell.2015.09.038 (25 September 2015)

An integrated map of structural variation in 2,504 human genomes

Sudmant, P.H. et al. Nature 526, 75–81 (2015)

Plasma DNA tissue mapping by genome-wide methylation sequencing for noninvasive prenatal, cancer, and transplantation assessments

Sun, K. et al. PNAS 112, E5503–E5512 (21 September 2015)

Sca/eS: an optical clearing palette for biological imaging

Hama, H. et al. Nat. Neurosci. 18, 1518–1529 (2015)

Genetic determinants of in vivo fitness and diet responsiveness in multiple human gut Bacteroides

Wu, M. et al. Science 350, 6256 (2015)