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Inflammatory characteristics of adipose tissue collected by surgical excision vs needle aspiration

International Journal of Obesity volume 39, pages 874–876 (2015)Cite this article

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Abstract

Subcutaneous adipose tissue can be obtained for research during an elective, clinically indicated operation by standard surgical excision approaches and by needle aspiration in pure research settings. Whether measurements of inflammatory markers and cells from tissues collected in these two different ways are comparable is debatable. We sought to determine whether these two techniques yield systematically different results for measurements of inflammation, cellular senescence and adipose tissue composition. Twelve subjects undergoing surgery participated. At the time of surgery abdominal subcutaneous adipose tissue from adjacent sites was removed by excision and needle aspiration. Stromovascular cell composition (flow cytometry), the number of senescent cells (senescence-associated-β-galactosidase staining) and interleukin (IL)-6, IL-1, TNF-α and MCP1 mRNA (reverse transcription-PCR) were measured in each sample. We found no statistically significant differences between the two sample-collection approaches for any of the parameters measured. We conclude that these two methods of obtaining adipose tissue do not systematically differ in the results of cytokine mRNA content, cellular senescence or stromovascular cell composition.

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Acknowledgements

This work was supported by National Institutes of Health grants DK-45343, DK-40484, DK-50456 and a NSERC Discovery Grant. Sylvia Santosa is a CRC Tier II, Clinical Nutrition.

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Authors and Affiliations

  1. Endocrine Research Unit, Mayo Clinic, Rochester, MN, USA

    S Santosa & M D Jensen

  2. Department of Exercise Science, Concordia University, Montreal, Québec, Canada,

    S Santosa

  3. Department of Surgery, Mayo Clinic, Rochester, MN, USA

    J Swain

  4. Scottsdale Healthcare Bariatric Center, Scottsdale, AZ, USA

    J Swain

  5. Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN, USA

    T Tchkonia & J L Kirkland

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  1. S Santosa
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  2. J Swain
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Correspondence to M D Jensen.

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The authors declare no conflict of interest.

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Santosa, S., Swain, J., Tchkonia, T. et al. Inflammatory characteristics of adipose tissue collected by surgical excision vs needle aspiration. Int J Obes 39, 874–876 (2015). https://doi.org/10.1038/ijo.2014.185

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