Abstract
In this study, we evaluated the efficacy of a novel method of seminal vesicle (SV) preparation—ring preparation method—in isolated SV experiments in the rat. Rat SVs were prepared as strips and rings and applied to organ baths. The relaxation responses by sodium nitroprusside or doxazosin and contractile responses by electrical field stimulation (EFS) were recorded in both groups. We compared the proportion of tissues that showed consistent responses to the stimuli—drug or EFS—in both groups. And magnitudes of the contractile or relaxation responses were also evaluated in the two groups. In strip preparations (n=20), six tissues (30%) showed stable responses to drugs and were regarded to be useful. However, in ring preparations (n=20), 13 tissues (65%) showed stable responses (P<0.001). Similarly, consistent contractile responses to the EFS were recorded in eight strips (40%) and 13 rings (65%) (n=20 in each group; P<0.05). The magnitudes of the relaxations by drugs were not different between the two methods (P=0.42) and the contractions by EFS were higher in ring preparations (P<0.05). The ring preparation seems to be better for responses to drugs and EFS compared with the strip preparations and, therefore, might be an alternative technique for in vitro SV experiments.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 8 print issues and online access
$259.00 per year
only $32.38 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Stjernquist M, Hakanson R, Leander S, Owman C, Sundler F, Uddman R . Immunohistochemical localization of substance P, vasoactive intestinal polypeptide and gastrin-releasing peptide in vas deferens and seminal vesicle, and the effect of these and eight other neuropeptides on resting tension and neurally evoked contractile activity. Regul Pept 1983; 7: 67–86.
Williams-Ashman HG . Regulatory features of seminal vesicle development and function. Curr Top Cell Regul 1983; 22: 201–275.
Turek PJ, Aslam K, Younes AK, Nguyen HT . Observations on seminal vesicle dynamics in an in vivo rat model. J Urol 1998; 159: 1731–1734.
McMahon CG . The etiology and management of premature ejaculation. Nat Clin Pract Urol 2005; 2: 426–433.
Salonia A, Maga T, Colombo R, Scattoni V, Briganti A, Cestari A et al. A prospective study comparing paroxetine alone versus paroxetine plus sildenafil in patients with premature ejaculation. J Urol 2002; 168: 2486–2489.
Nudell DM, Monoski MM, Lipshultz LI . Common medications and drugs: how they affect male fertility. Urol Clin North Am 2002; 29: 965–973.
Giuliano F, Bernabe J, Droupy S, Alexandre L, Allard J . A comparison of the effects of tamsulosin and alfuzosin on neurally evoked increases in bladder neck and seminal vesicle pressure in rats. BJU Int 2004; 93: 605–608.
Orhan I, Onur R, Tasdemir C, Ayar A, Kadioglu A . Sildenafil citrate inhibits agonist induced contractions in isolated rat seminal vesicles. J Urol 2006; 175: 2350–2353.
Machtens S, Ckert S, Stief CG, Tsikas D, Frlich JC, Jonas U . Effects of various nitric oxide-donating drugs on adrenergic tension of human seminal vesicles in vitro. Urology 2003; 61: 479–483.
Gokhale SD, Bashir AA, Chandranath SI . Functional responses of the rat isolated seminal vesicle to electrical field stimulation: a pharmacological analysis. Clin Exp Pharmacol Physiol 1996; 23: 22–29.
Rasmussen H, Forder J, Kojima I, Scriabine A . TPA-induced contraction of isolated rabbit vascular smooth muscle. Biochem Biophys Res Commun 1984; 122: 776–784.
Medina P, Segarra G, Vila JM, Domenech C, Martinez-Leon JB, Lluch S . Effects of sildenafil on human penile blood vessels. Urology 2000; 56: 539–543.
Carson C, Gunn K . Premature ejaculation: definition and prevalence. Int J Impot Res 2006; 18 (Suppl 1): S5–13.
Perelman M, McCullough AR, Bull S, Ho KF, Jamieson CJ . The impact of self-reported premature ejaculation on other aspects of male sexual function. J Sex Med 2004; 1 (Suppl 1): aS59.
Donatucci CF . Etiology of ejaculation and pathophysiology of premature ejaculation. J Sex Med 2006; 3 (Suppl 4): 303–308.
Waring JV, Wendt IR . Effects of streptozotocin-induced diabetes mellitus on intracellular calcium and contraction of longitudinal smooth muscle from rat urinary bladder. J Urol 2000; 163: 323–330.
Ding AQ, Stallone JN . Testosterone-induced relaxation of rat aorta is androgen structure specific and involves K+ channel activation. J Appl Physiol 2001; 91: 2742–2750.
Johnson CP, How T, Scraggs M, West CR, Burns J . A biomechanical study of the human vertebral artery with implications for fatal arterial injury. Forensic Sci Int 2000; 109: 169–182.
Sharif SI . Dopamine contracts the rat isolated seminal vesicle by activation of postjunctional alpha-1-adrenoceptors. Pharmacology 1994; 48: 328–334.
Silva MA, Megale A, Avellar MC, Porto CS . Expression and pharmacological characterization of alpha1-adrenoceptors in rat seminal vesicle. Eur J Pharmacol 1999; 381: 141–149.
Somlyo AP, Somlyo AV . Signal transduction by G-proteins, rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II. J Physiol 2000; 522 (Part 2): 177–185.
Heuer O, Uckert S, Machtens SA, Stief CG, Tsikas D, Frolich JC et al. Effects of various nitric oxide donating agents on the contractility and cyclic nucleotide turnover of human seminal vesicles in vitro. Urology 2002; 59: 958–962.
Simonsen U, Garcia-Sacristan A, Prieto D . Penile arteries and erection. J Vasc Res 2002; 39: 283–303.
Sadraei H, Large BJ, Hughes IE . Mechanisms involved in electrically-induced responses of rat seminal vesicles. J Pharm Pharmacol 1995; 47: 665–668.
Fedan JS, Besse JC, Carpenter FG, Teague RS . Motor innervation of the smooth muscle of the rat seminal vesicle. J Pharmacol Exp Ther 1977; 201: 285–297.
Moss HE, Crowe R, Burnstock G . The seminal vesicle in eight and 16 week streptozotocin-induced diabetic rats: adrenergic, cholinergic and peptidergic innervation. J Urol 1987; 138: 1273–1278.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, H., Jeong, J., Yang, J. et al. A novel method of seminal vesicle preparation in isolated seminal vesicle experiments in the rat: ring preparation. Int J Impot Res 21, 57–61 (2009). https://doi.org/10.1038/ijir.2008.57
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/ijir.2008.57