Abstract
In chronically HIV infected individuals, a number of functional B cell abnormalities have been described. However, the immediate changes that occur in the B cell compartment following viral exposure and how they affect the long-term course of infection are not well understood. We report the longitudinal analysis of B cell repertoires during early infection in untreated and treated individuals receiving highly active antiretroviral therapy (HAART). Analysis was based on IgG heavy chain gene utilization and CDR3 length measurement and relationship with CD4/CD8 counts, viral load, and total serum IgG, and anti-HIV antibodies levels. Repertoires were assessed at baseline and at weeks 2, 4, 12, 24, and 72 after initiation of therapy. The findings indicate a stable peripheral B cell repertoire during the first 72 weeks following infection, particularly in the HAART treated patients. A modest association between B cell repertoire integrity and viremia levels as well as treatment was detected.
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Acknowledgements
We thank the study participants. We acknowledge the assistance of Gerald Spotts with database and data management. This study was supported by NIH 1UO1 AI41531, UCSF Center for AIDS Research NIH P30 MH59037.
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Elkins, M., Vittinghoff, E., Baranzini, S. et al. Longitudinal analysis of B cell repertoire and antibody gene rearrangements during early HIV infection. Genes Immun 6, 66–69 (2005). https://doi.org/10.1038/sj.gene.6364146
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DOI: https://doi.org/10.1038/sj.gene.6364146
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