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Targeted disruption in Arabidopsis

Nature volume 389, pages 802–803 (1997)Cite this article

Abstract

Homologous recombination has been used for two decades to target insertions into cloned genes in bacteria and yeast, and more recently has become a routine method of gene inactivation in mammals. Arabidopsis is one of several multicellular model organisms (along with Drosophila, Caenorhabditis and zebrafish) in which mechanisms controlling development have been studied. Previously, traditional genetic methods have been used, as targeted disruption by homologous recombination has not been successful in any of these organisms. We have now successfully disrupted the AGL5 MADS-box gene in Arabidopsisby homologous recombination, providing a useful tool for future analyses.

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Figure 1: Diagram of wild-type AGL5 genomic region, knockout (KO) construct, and agl5 mutant genomic region.
Figure 2: Genetic analysis of agl5mutant.

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Authors and Affiliations

  1. Department of Biology, Center for Molecular Genetics, University of California at San Diego, La Jolla, 92093-0116, California, USA

    Sherry A. Kempin, Sarah J. Liljegren, Laura M. Block, Steven D. Rounsley & Martin F. Yanofsky

  2. AgBiotech Center, Foran Hall, Dudley Road, Rutgers the State University of New Jersey, New Brunswick, 08903, New Jersey, USA

    Eric Lam

Authors
  1. Sherry A. Kempin
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  2. Sarah J. Liljegren
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  3. Laura M. Block
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  4. Steven D. Rounsley
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  5. Martin F. Yanofsky
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  6. Eric Lam
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Kempin, S., Liljegren, S., Block, L. et al. Targeted disruption in Arabidopsis. Nature 389, 802–803 (1997). https://doi.org/10.1038/39770

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