The use of a thermally activated DNA polymerase PCR gives improved specificity, sensitivity and product yield without additives or extra process steps.
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Birch, D., Kolmodin, L., Wong, J. et al. Simplified hot start PCR. Nature 381, 445–446 (1996). https://doi.org/10.1038/381445a0
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DOI: https://doi.org/10.1038/381445a0