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Yeast TAF IIS in a multisubunit complex required for activated transcription

Nature volume 371, pages 523–527 (1994)Cite this article

Abstract

IN higher eukaryotes the RNA polymerase II transcription factor TFIID is composed of a TATA-box-binding protein (TBP) and a set of tightly bound polypeptides, designated TBP-associated factors (TAFIIS). One or more TAFIIS are coactivators that are required for activated but not basal transcription1–3. The eukary-otic transcription machinery is highly conserved and it is therefore puzzling that TAFIIS have not been identified in yeast. Here we use TBP as a protein-affinity ligand to isolate from yeast a multisubunit complex that is required specifically for activated transcription by RNA polymerase II. Microsequence analysis and cloning of two subunits of this complex reveal that they are the homologues of known mammalian and Drosophila TAFIIS. The genes encoding these two yeast TAFIIS are essential, suggesting that activated transcription is required for viability of Saccharo-myces cerevisiae.

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Authors and Affiliations

  1. Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical Center, 373 Plantation Street, Worcester, Massachusetts, 01605, USA

    Joseph C. Reese, Lynne Apone, Scott S. Walker, Loree A. Griffin & Michael R. Green

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  1. Joseph C. Reese
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  2. Lynne Apone
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  3. Scott S. Walker
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  4. Loree A. Griffin
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  5. Michael R. Green
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Reese, J., Apone, L., Walker, S. et al. Yeast TAF IIS in a multisubunit complex required for activated transcription. Nature 371, 523–527 (1994). https://doi.org/10.1038/371523a0

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