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Cloning and functional expression of a rat heart KATP channel

Nature volume 370, pages 456–459 (1994)Cite this article

A Retraction to this article was published on 28 December 1995

Abstract

POTASSIUM channels that are ATP-sensitive (KATP) couple membrane potential to the metabolic status of the cell. KATP channels are inhibited by intracellular ATP and are stimulated by intracellular nucleotide diphosphates1. KATP channels are important regulators of secretory processes and muscle contraction, and are targets for therapeutic treatment of type II diabetes by the inhibitory sulphonylureas2 and for hypertension by activators such as pinacidil3. In cardiac tissue, KATP channels are central regulators of post-ischaemic cardioprotection4,5. Electrophysiological and pharmacological characteristics vary among KATP channels recorded from diverse tissues suggesting extensive molecular heterogeneity1. A complementary DNA encoding a KATP channel was isolated from rat heart using the polymerase chain reaction. We report here that the expressed channels possess all of the essential features of native cardiac KATP channels, including sensitivity to intracellular nucleotides. In addition the cloned channels are activated by the potassium channel opener, pinacidil, but are not inhibited by the sulphonylurea, glibenclamide.

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Authors and Affiliations

  1. Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QJ, UK

    M. L. J. Ashford

  2. Vollum Institute, Oregon Health Sciences University, Portland, Oregon, 97201, USA

    C. T. Bond, T. A. Blair & J. P. Adelmair

Authors
  1. M. L. J. Ashford
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  2. C. T. Bond
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  3. T. A. Blair
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  4. J. P. Adelmair
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Ashford, M., Bond, C., Blair, T. et al. Cloning and functional expression of a rat heart KATP channel. Nature 370, 456–459 (1994). https://doi.org/10.1038/370456a0

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