Abstract
MANY hormones or neurotransmitters act at cell surface receptors to increase the intracellular free calcium concentration, triggering a wide range of cellular responses1. As the source of this Ca2+ is often internal stores, additional messengers are required to convey the hormonal message from the plasma membrane. Cyclic ADP-ribose (cADPR) has been proposed as the endogenous activator of Ca2+-induced Ca2+ release by the ryanodine receptor in sea urchin eggs and in several mammalian cell types2–8,22. A second messenger role for cADPR requires that its intracellular levels be under the control of extracellular stimuli. Here we demonstrate a novel action of 3',5'-cyclic guanosine monophosphate (cGMP) in stimulating the synthesis of cADPR from β-NAD+ by activating its synthetic enzyme ADP-ribosyl cyclase9–11 in sea urchin eggs and egg homogenates. We suggest that cADPR may transduce signals generated by cell surface receptors or gaseous transmitters linked to cGMP production.
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References
Berridge, M. J. Nature 361, 315–325 (1993).
Galione, A., Lee, H. C. & Busa, W. B. Science 253, 1143–1146 (1991).
Galione, A. Trends pharmac. Sci. 13, 504–506 (1992).
Currie, K., Swann, K., Galione, A. & Scott, R. H. Molec. Biol. Cell 3, 1415–1422 (1992).
Takesawa, S., Nata, K., Yonekura, H. & Okamoto, H. Science 259, 370–374 (1993).
Galione, A. Science 259, 325–326 (1993).
Lee, H. C. J. biol. Chem. 268, 293–299 (1993).
White, A. M., Watson, S. P. & Galione, A. FEBS Lett. 318, 259–263 (1993).
Ruskino, N. & Lee, H. C. J. biol. Chem. 264, 11725–11731 (1989).
Lee, H. C. & Aarhus, R. Cell Reg. 2, 203–209 (1991).
Hellmich, M. R. & Strumwasser, F. Cell Reg. 2, 193–202 (1991).
Dargie, P. J., Agre, M. C. & Lee, H. C. Cell Reg. 1, 279–290 (1990).
Potter, B. V. L. in Intracellular Messengers; Neuromethods Vol. 20 (eds Boulton, A. A., Baker, G. B. & Taylor, C. W.) 273–312 (Humana, Totowa, New Jersey, 1992).
Butt, E., van Bemmelen, M., Fischer, L., Walter, U. & Jastorff, B. FEBS Lett. 263, 47–50 (1990).
Whalley, T., McDougall, A., Crossley, I., Swann, K. & Whitaker, M. Molec. Biol. Cell 3, 373–383 (1992).
Hofmann, F., Gensheimer, H.-P., Landgraf, W., Hullin, R. & Jastorff, B. Eur. J. Biochem. 150, 85–88 (1985).
Whitaker, M. J., Swann, K. & Crossley, I. B. in Mechanisms of Egg Activation (ed. Nuccitelli, R.) 159–163 (Plenum, New York, 1989).
Swann, K. S., Ciapa, B. & Whitaker, M. J. in Molecular Biology of Invertebrate Development (ed. O'Connor, D.) 45–69 (Liss, New York, 1987).
Whitaker, M. J. & Swann, K. Development 117, 1–12 (1993).
Jaffe, L. F. Proc. natn. Acad. Sci. U.S.A. 88, 9883–9887 (1991).
Poenie, M. Cell Calcium 11, 85–91 (1990).
Mészáros, L. G., Bak, J. & Chu, A. Nature 364, 76–79 (1993).
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Galione, A., White, A., Willmott, N. et al. cGMP mobilizes intracellular Ca2+ in sea urchin eggs by stimulating cyclic ADP-ribose synthesis. Nature 365, 456–459 (1993). https://doi.org/10.1038/365456a0
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DOI: https://doi.org/10.1038/365456a0
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